Semi-Automatic Rating Method for Neutrophil Alkaline Phosphatase Activity
نویسندگان
چکیده
منابع مشابه
Neutrophil alkaline phosphatase in pregnancy.
Neutrophil alkaline phosphatase levels have been studied in a group of 194 pregnant women, using a modified technique which permits more rapid counting of the required number of neutrophils with a degree of consistency comparable with standard methods. No correlation was found between neutrophil alkaline phosphatase levels and the period of gestation; in contrast to previous findings, no differ...
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LTHOUGH THE LEVEL of circulating serum enzyme activity may not in many instances represent occurrences at the cellular level, there has been a consistent increase in the number of requests for clinical serum enzyme determinations. There has also been an increase in the number of types of enzyme activity requested, making it imperative for clinical chemistry laboratories to employ the most effic...
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The potential use of the protein denaturant guanidine hydrochloride to inhibit selectively the enzyme activity of serum alkaline phosphatase isoenzymes from liver, bone, intestine, and placenta was investigated. Inhibition of each isoenzyme was shown to be dependent on time and concentration of inhibitor. In the presence of 0-3 mol/l (28.7 g/l) guanidine hydrochloride for 170 seconds 14%, 47%, ...
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To determine whether decreased alkaline phosphatase activity in the granules from neutrophils of patients with chronic myelogenous leukemia (CML) was due to an absence of enzyme or the production of defective enzyme, we compared the immunologic properties of granule alkaline phosphatase derived from patients with CML with that of normal subjects and patients with polycythemia vera (PRy). Antise...
متن کاملQuantitative method for determining serum alkaline phosphatase isoenzyme activity II. Development and clinical application of method for measuring four serum alkaline phosphatase isoenzymes.
A method for quantitating the liver, bone, intestinal and placental alkaline phosphatase activity of serum, using an algorithm for converting selective inactivation by guanidine hydrochloride, L-phenylalanine, and heat into equivalent isoenzyme activity is described. The method can individually quantify mixtures of isoenzymes to within a margin of 3%; it has acceptable reproducibility and has b...
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ژورنال
عنوان ژورنال: Journal of Clinical Laboratory Analysis
سال: 2016
ISSN: 0887-8013
DOI: 10.1002/jcla.22022